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Effects of two peptides on seizures in the Zebrafish PTZ model

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dc.contributor.author Araujo Fernández, Jhonathan Angel
dc.contributor.author Moura, Thatiane Cristina de
dc.contributor.author Sentis, Marco Eugenio Vazquez
dc.contributor.author Santos, Maria D. Mayán
dc.contributor.author Piñón, Laura Elena Sánchez
dc.contributor.author Maurer-Morelli, Cláudia Vianna
dc.date 2024-10-18
dc.date.accessioned 2024-10-17
dc.date.accessioned 2024-10-20T01:11:22Z
dc.date.available 2024-10-20T01:11:22Z
dc.identifier.uri https://doi.org/10.25824/redu/4TJY6C
dc.identifier.uri https://redu.unicamp.br/dataset.xhtml?persistentId=doi:10.25824/redu/4TJY6C
dc.description In this study, we used the zebrafish PTZ-seizure model to investigate the effects of two peptides on seizure suppression: the tripeptide p-BTX-I (AminoTech) and the Cx43 peptide CX2 (PCT/EP2020/071242). Wild-type zebrafish larvae at 6 days post-fertilization were exposed to one of the peptides, according to their group, for 24 hours before PTZ-induced seizure induction. We quantified the expression of genes related to inflammation, oxidative stress, and apoptosis by RT-qPCR, using SYBR® Green Master Mix reagent (Bio-Rad) in the ABI 7500 system (Applied Biosystems, Foster City, CA, USA). The runs were performed in triplicate, with eef1a1l1 used as the housekeeping gene. Additionally, we quantified seizure latency and frequency through visual observation. Here, we present the data sheet and videos used for the assessment of seizure behavior.
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorship ACUIGEN
dc.description.sponsorship Xunta de Galicia
dc.description.sponsorship Xunta de Galicia
dc.description.sponsorship MCIN/AEI/10.13039/501100011033 and by ERDF A way of making Europe
dc.description.sponsorship Centro singular de Investigación de Galicia accreditation 2016–2019
dc.description.sponsorship Centro singular de Investigación de Galicia accreditation 2016–2019
dc.format video/mp4
dc.format video/mp4
dc.format text/plain
dc.format text/tab-separated-values
dc.format video/mp4
dc.format video/mp4
dc.format text/plain
dc.publisher Vianna Maurer Morelli, Cláudia
dc.subject Medicine, Health and Life Sciences
dc.title Effects of two peptides on seizures in the Zebrafish PTZ model
dc.description.sponsorshipId FAPESP: 2013-07559-3
dc.description.sponsorshipId CAPES: 88887.470075/2019-00
dc.description.sponsorshipId CAPES: 001
dc.description.sponsorshipId GI-1251
dc.description.sponsorshipId ED431C 2018/28
dc.description.sponsorshipId ED431B 2018/04
dc.description.sponsorshipId PID2021-127702NB-I00
dc.description.sponsorshipId ED431G/09
dc.description.sponsorshipId ED431C2021/29


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