| dc.contributor.author |
Araujo Fernández, Jhonathan Angel |
|
| dc.contributor.author |
Moura, Thatiane Cristina de |
|
| dc.contributor.author |
Sentis, Marco Eugenio Vazquez |
|
| dc.contributor.author |
Santos, Maria D. Mayán |
|
| dc.contributor.author |
Piñón, Laura Elena Sánchez |
|
| dc.contributor.author |
Maurer-Morelli, Cláudia Vianna |
|
| dc.date |
2024-10-18 |
|
| dc.date.accessioned |
2024-10-17 |
|
| dc.date.accessioned |
2024-10-20T01:11:22Z |
|
| dc.date.available |
2024-10-20T01:11:22Z |
|
| dc.identifier.uri |
https://doi.org/10.25824/redu/4TJY6C |
|
| dc.identifier.uri |
https://redu.unicamp.br/dataset.xhtml?persistentId=doi:10.25824/redu/4TJY6C |
|
| dc.description |
In this study, we used the zebrafish PTZ-seizure model to investigate the effects of two peptides on seizure suppression: the tripeptide p-BTX-I (AminoTech) and the Cx43 peptide CX2 (PCT/EP2020/071242). Wild-type zebrafish larvae at 6 days post-fertilization were exposed to one of the peptides, according to their group, for 24 hours before PTZ-induced seizure induction. We quantified the expression of genes related to inflammation, oxidative stress, and apoptosis by RT-qPCR, using SYBR® Green Master Mix reagent (Bio-Rad) in the ABI 7500 system (Applied Biosystems, Foster City, CA, USA). The runs were performed in triplicate, with eef1a1l1 used as the housekeeping gene. Additionally, we quantified seizure latency and frequency through visual observation. Here, we present the data sheet and videos used for the assessment of seizure behavior. |
|
| dc.description.sponsorship |
Fundação de Amparo à Pesquisa do Estado de São Paulo |
|
| dc.description.sponsorship |
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
|
| dc.description.sponsorship |
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
|
| dc.description.sponsorship |
ACUIGEN |
|
| dc.description.sponsorship |
Xunta de Galicia |
|
| dc.description.sponsorship |
Xunta de Galicia |
|
| dc.description.sponsorship |
MCIN/AEI/10.13039/501100011033 and by ERDF A way of making Europe |
|
| dc.description.sponsorship |
Centro singular de Investigación de Galicia accreditation 2016–2019 |
|
| dc.description.sponsorship |
Centro singular de Investigación de Galicia accreditation 2016–2019 |
|
| dc.format |
video/mp4 |
|
| dc.format |
video/mp4 |
|
| dc.format |
text/plain |
|
| dc.format |
text/tab-separated-values |
|
| dc.format |
video/mp4 |
|
| dc.format |
video/mp4 |
|
| dc.format |
text/plain |
|
| dc.publisher |
Vianna Maurer Morelli, Cláudia |
|
| dc.subject |
Medicine, Health and Life Sciences |
|
| dc.title |
Effects of two peptides on seizures in the Zebrafish PTZ model |
|
| dc.description.sponsorshipId |
FAPESP: 2013-07559-3 |
|
| dc.description.sponsorshipId |
CAPES: 88887.470075/2019-00 |
|
| dc.description.sponsorshipId |
CAPES: 001 |
|
| dc.description.sponsorshipId |
GI-1251 |
|
| dc.description.sponsorshipId |
ED431C 2018/28 |
|
| dc.description.sponsorshipId |
ED431B 2018/04 |
|
| dc.description.sponsorshipId |
PID2021-127702NB-I00 |
|
| dc.description.sponsorshipId |
ED431G/09 |
|
| dc.description.sponsorshipId |
ED431C2021/29 |
|