DSpace/Manakin Repository

Integrated imaging and molecular biology dataset for the characterization of a putative anti-eukaryotic effector from the Xanthomonas citri type VI secretion system

Mostrar registro simples

dc.contributor.author Santos, José Felipe Teixeira da Silva
dc.contributor.author Alvarez-Martinez, Cristina Elisa
dc.date 2026-01-19
dc.date.accessioned 2025-12-22
dc.date.accessioned 2026-01-21T01:12:07Z
dc.date.available 2026-01-21T01:12:07Z
dc.identifier.uri https://doi.org/10.25824/redu/VVH03L
dc.identifier.uri https://redu.unicamp.br/dataset.xhtml?amp;persistentId=doi:10.25824/redu/VVH03L
dc.description This dataset comprises integrated quantitative and qualitative experimental data generated for the characterization of a putative anti-eukaryotic effector associated with the Type VI Secretion System (T6SS) of Xanthomonas citri subsp. citri. The data were obtained using a combination of molecular biology, biochemical, NGS, and imaging-based approaches, including blotting techniques, and microscopy. Quantitative data include measurements such as area, time, signal intensity, and relative abundance of biological molecules, while qualitative data consist of microscopy images and experimental videos. Experiments were conducted using the bacterial model X. citri subsp. citri and the eukaryotic model organisms Dictyostelium discoideum and Saccharomyces cerevisiae, enabling the assessment of effector activity across different biological systems. Whenever applicable, experiments were performed in biological triplicates to ensure statistical robustness. Data acquisition was carried out using multi-user equipment from the Institute of Biology at the University of Campinas (IB-UNICAMP), with additional use of facilities like National Institute of Photonics Applied to Cell Biology (INFABiC), Laboratório Central de Tecnologias de Alto Desempenho em Ciências da Vida (LaCTAD), and NGS soluções genômicas. Metadata associated with the dataset include title, subject, author information, abstract, technical specifications of the equipment used, keywords, and persistent identifiers, following the DataCite Metadata Schema standard. The dataset was stored in multiple file formats according to data type, including spreadsheets (XLS/XLSX), tabulated text files (TXT, CSV, TSV), image and video formats (JPEG, TIFF, PNG, MP4, WMV, OGV), and statistical analysis files (RData). The data cannot be made available for intellectual property reasons. For more information, please contact those responsible.
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.publisher Teixeira Da Silva Santos, José Felipe
dc.subject Earth and Environmental Sciences
dc.subject Medicine, Health and Life Sciences
dc.title Integrated imaging and molecular biology dataset for the characterization of a putative anti-eukaryotic effector from the Xanthomonas citri type VI secretion system
dc.description.sponsorshipId FAPESP: 2022/11998-1
dc.description.sponsorshipId FAPESP: 2024/05826-9
dc.description.sponsorshipId FAPESP: 2022/06555-3
dc.description.sponsorshipId CAPES: 001


Arquivos deste item

Arquivos Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item aparece na(s) seguinte(s) coleção(s)

Mostrar registro simples