This dataset explores the role of spinal cord reactive oxygen species (ROS) in regulating sympathetic vasomotor activity in Goldblatt hypertensive rats (experimental renovascular hypertension). Hypertension was induced by clipping the renal artery, and six weeks later, a catheter was inserted into the subarachnoid space at the T10-11 vertebral level in urethane-anaesthetized rats. The effects of intrathecal Tempol injection on mean arterial pressure (MAP), heart rate (HR), renal sympathetic nerve activity (rSNA), and splanchnic sympathetic nerve activity (sSNA) were recorded over 60 minutes. Additionally, mRNA expression of oxidative balance enzymes was quantified in the spinal cord, and ROS levels were assessed using the DHE fluorescence method.