This dataset contains raw experimental data acquired using multiple analytical techniques, including single-cell ICP-MS and FRET-based methods. It encompasses trace metal profiling primarily zinc and iron in biological systems, focusing on intracellular distribution, uptake mechanisms, and protein-metal interactions. The data were collected under a variety of experimental conditions, including disease-related models. A subset of the dataset specifically explores metal homeostasis in biological samples from individuals with and without COVID-19, supporting investigations into the role of metals in infection and immune response.