Objective of the study: to evaluate the effect of different storage temperatures of a 10% carbamide peroxide (CP) gel on bleaching efficacy and enamel surface roughness by using bovine incisor specimens (4 x 4 x 3mm) who were randomized and allocated to five study groups (n=10): bleaching with bleaching gel (Whiteness Perfect 10%, FGM) without prior storage (WP, positive control), gels stored for three months at temperatures of 25ºC (WP 3m25ºC), 35°C (WP 3m35ºC), and 41°C (WP 3m41ºC), and specimens kept in remineralizing solution (NT, negative control). Color analyses (ΔE , ΔE00 and ΔWID) and roughness (Ra, µm) were performed considering the initial time (T1) and 24 hours after the fourteenth day of bleaching (T2).