Characterizing the tumor microenvironment (TME) is vital for understanding the intricate cellular crosstalk within tumors and for identifying critical immunological markers. Given the TME's contribution to neoplasm development and progression, a comprehensive analysis can significantly advance cancer diagnosis, prognosis, and therapeutic interventions. Dysregulation of WNT signaling, notably WNT5A, is a key driver of tumorigenesis in multiple cancers. WNT5A, an evolutionarily conserved protein, displays context-dependent oncogenic or tumor-suppressive activities through modulation of non-canonical pathways, affecting cell growth, migration, and invasion. Therefore, understanding the role of this marker in different neoplasms, particularly hematological malignancies, is crucial for developing targeted therapies and improving patient outcomes.