This dataset includes data from a manuscript published in the journal Nature Communications, entitled "An Intricate Network Involving the Argonaute ALG-1 Modulates Organismal Resistance to Oxidative Stress." It contains Excel spreadsheets and data presented as charts, organized by experiment type. These files include the results of experiments conducted to investigate the role of the Argonaute protein ALG-1 in promoting healthy aging and responding to oxidative stress in C. elegans. The data encompasses lifespan assays, oxidative stress resistance experiments, and molecular and imaging analyses of transcriptional and post-transcriptional regulatory pathways involving ALG-1.