This study aimed to investigate tissue repair of excisional wounds of hyperglycemic animals treated with chitosan membrane and alginate produced in the presence of glycerol. The 8-week-old C57Bl male mice were divided into normoglycemic animals and topical treatment with 0.9% saline solution (CTSF); hyperglycemic animals and topical treatment with 0.9% saline solution (DMSF) and hyperglycemic animals and topical treatment with chitosan membrane and alginate (McMC). On the 3rd postoperative day, the McMC group presented a lower number of leukocytes, mature mast cells, the number of vessels (p<0.05) and the number of active mast cells (p<0.05) compared to the other GROUPS CTSF and DMSF. No differences in the distribution, deposition, organization and thickness of collagen fibers. On the 7th day there was no difference in the analysis of fibroblasts, mast cells, and expression of TGF-b1 and VEGF between the groups. For collagen fibers, the DMCAM group presented mild red-orange birefringence compared to the CTSF and DMSF groups. On the 14th day there was a small concentration of thinner elastic fibers for the DMCAM group, with better reorganized papillary dermis and collagen fibers with better red-orange birefringence and network-shaped orientation, similar to intact skin, with better organization of elastic fibers, sparse throughout the neoderm and with greater presence of elauninic fibers, similar to the elastic fibers of intact skin. The use of CAM in skin lesions favored tissue repair, through a smaller number of inflammatory cells and mast cells, improving the deposition of collagen and collagen fibers. Demonstrating potential coverage for skin wounds.